Crispr/Cas9 editing reveals novel mechanisms of clustered microRNA regulation and function
- Submitting institution
-
Middlesex University
- Unit of assessment
- 11 - Computer Science and Informatics
- Output identifier
- 1500
- Type
- D - Journal article
- DOI
-
10.1038/s41598-017-09268-0
- Title of journal
- Scientific Reports
- Article number
- 8585
- First page
- 1
- Volume
- 7
- Issue
- -
- ISSN
- 2045-2322
- Open access status
- Compliant
- Month of publication
- August
- Year of publication
- 2017
- URL
-
http://eprints.mdx.ac.uk/22359/
- Supplementary information
-
-
- Request cross-referral to
- -
- Output has been delayed by COVID-19
- No
- COVID-19 affected output statement
- -
- Forensic science
- No
- Criminology
- No
- Interdisciplinary
- No
- Number of additional authors
-
9
- Research group(s)
-
-
- Citation count
- 14
- Proposed double-weighted
- No
- Reserve for an output with double weighting
- No
- Additional information
- This paper focused on different regulatory mechanisms in miRNA cluster expression showing that CRISPR/Cas9 is a powerful tool to dissect the regulation and function of clustered microRNAs. Computational support for understanding 3D structure and function of microRNAs, microRNA clusters and genes supports understanding and search of the large gene function space. This paper is significant because the technology provides a unique platform to establish mutant cell lines or mouse models and to elucidate the specific contribution of miRNA families in cellular responses both at baseline and following injury.
- Author contribution statement
- -
- Non-English
- No
- English abstract
- -