A comparative assessment of conventional and molecular methods, including MinION nanopore sequencing, for surveying water quality
- Submitting institution
-
University of Newcastle upon Tyne
- Unit of assessment
- 12 - Engineering
- Output identifier
- 261261-59344-1293
- Type
- D - Journal article
- DOI
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10.1038/s41598-019-51997-x
- Title of journal
- Scientific Reports
- Article number
- 15726
- First page
- -
- Volume
- 9
- Issue
- -
- ISSN
- 2045-2322
- Open access status
- Compliant
- Month of publication
- October
- Year of publication
- 2019
- URL
-
https://doi.org/10.1038/s41598-019-51997-x
- Supplementary information
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-
- Request cross-referral to
- -
- Output has been delayed by COVID-19
- No
- COVID-19 affected output statement
- -
- Forensic science
- No
- Criminology
- No
- Interdisciplinary
- No
- Number of additional authors
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5
- Research group(s)
-
-
- Proposed double-weighted
- No
- Reserve for an output with double weighting
- No
- Additional information
- This is the first evaluation of the MinION, a memory-stick sized next generation sequencing device from project partner Oxford Nanopore Technologies, applied to microbial drinking water quality monitoring. It evaluates the MinION with a sample of known composition and demonstrates superior genus and species level taxonomic resolution compared to the "gold standard" non-portable method (MiSeq of Illumina). This proof of concept has led to substantial funding from the Newton and Global Challenges Research Funds and development of a suitcase laboratory which now enables partners in Tanzania, Ethiopia, Nepal and Malaysia to do state-of-the-art water microbiology.
- Author contribution statement
- -
- Non-English
- No
- English abstract
- -