Real-time monitoring of DNA immobilization and detection of DNA polymerase activity by a microfluidic nanoplasmonic platform
- Submitting institution
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University of Ulster
- Unit of assessment
- 12 - Engineering
- Output identifier
- 77505420
- Type
- D - Journal article
- DOI
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10.1016/j.bios.2019.111528
- Title of journal
- Biosensors and Bioelectronics
- Article number
- 111528
- First page
- -
- Volume
- 142
- Issue
- -
- ISSN
- 0956-5663
- Open access status
- Compliant
- Month of publication
- July
- Year of publication
- 2019
- URL
-
-
- Supplementary information
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-
- Request cross-referral to
- -
- Output has been delayed by COVID-19
- No
- COVID-19 affected output statement
- -
- Forensic science
- No
- Criminology
- No
- Interdisciplinary
- No
- Number of additional authors
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4
- Research group(s)
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A - Healthcare Sensor Systems
- Proposed double-weighted
- No
- Reserve for an output with double weighting
- No
- Additional information
- This novel work has formed the fundamental basis of a new DNA sequencing technology. By combining microfluidics and gold nanomushrooms, binding events related to single nucleotide attachment are captured by localized surface plasmon resonance in nanostructures. The remarkable achievement was the ability to demonstrate DNA-enzyme interactions without the need of conventional heater for PCR reactions. These temperature requirements are fulfilled in a controlled manner by the plasma photothermal effect arising due to the interaction of light with the nanomushrooms. This concept can universally be applied to any complex reactions involving single nucleic acids, further driving innovations in biosensing technologies.
- Author contribution statement
- -
- Non-English
- No
- English abstract
- -