Automated high-throughput capillary circular dichroism and intrinsic fluorescence spectroscopy for rapid determination of protein structure
- Submitting institution
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The University of Birmingham
- Unit of assessment
- 12 - Engineering
- Output identifier
- 91841378
- Type
- D - Journal article
- DOI
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10.1021/acs.analchem.9b03259
- Title of journal
- Analytical Chemistry
- Article number
- -
- First page
- 13794
- Volume
- 91
- Issue
- 21
- ISSN
- 0003-2700
- Open access status
- Compliant
- Month of publication
- October
- Year of publication
- 2019
- URL
-
-
- Supplementary information
-
-
- Request cross-referral to
- -
- Output has been delayed by COVID-19
- No
- COVID-19 affected output statement
- -
- Forensic science
- No
- Criminology
- No
- Interdisciplinary
- Yes
- Number of additional authors
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4
- Research group(s)
-
-
- Proposed double-weighted
- No
- Reserve for an output with double weighting
- No
- Additional information
- Tangible evidence that biotherapeutic proteins are maintained in the correctly folded ‘active’ 3D-structure during manufacture is a vital Biopharma concern. This EPSRC/industry funded output describes the invention of an automated analytical instrument that uses tiny sample volumes to deliver ‘immediate’ assessments of a target molecule’s structure. It led to: two new industrial collaborations in 2020 employing the device to inform the manufacturing and formulation of monoclonal antibody therapeutics (EP/S023070/1; EngD studentship, Lonza) and in vitro transcribed mRNA vaccines (BB/V509371/1, £102k; Industrial CASE, AstraZeneca); three follow-on papers (https://doi.org/10.1016/j.chroma.2019.460429, and two in preparation); and Moore-Kelly’s appointment at Oxford Biomedica (Analytical Automation Specialist).
- Author contribution statement
- -
- Non-English
- No
- English abstract
- -