BRET-linked ATP assay with luciferase.
- Submitting institution
-
University of Cambridge
- Unit of assessment
- 12 - Engineering
- Output identifier
- 9838
- Type
- D - Journal article
- DOI
-
10.1039/c4an00436a
- Title of journal
- Analyst
- Article number
- -
- First page
- 4185
- Volume
- 139
- Issue
- 17
- ISSN
- 1364-5528
- Open access status
- Out of scope for open access requirements
- Month of publication
- -
- Year of publication
- 2014
- URL
-
-
- Supplementary information
-
-
- Request cross-referral to
- -
- Output has been delayed by COVID-19
- No
- COVID-19 affected output statement
- -
- Forensic science
- No
- Criminology
- No
- Interdisciplinary
- No
- Number of additional authors
-
1
- Research group(s)
-
-
- Proposed double-weighted
- No
- Reserve for an output with double weighting
- No
- Additional information
- Use of synthetic biology in protein fusion fixed the dipole interaction between a donor fluorescent protein and acceptor luciferin giving a novel wavelength shift assay featured as a journal cover page. The fusion approach led to a Royal Society International Award (IC160089 nucleic acid diagnostic) and an outline for local diagnostic enzyme production for low income countries (10.1016/j.biomaterials.2018.12.003). It was modified for a sarcosine biosensor testing prostate cancer (DOI: 10.1016/j.snb.2019.02.061) and the fused protein fluorescent detection is now core to an accessible nucleic acid test and enabled clinical trials in Ghana of malaria and a Covid testing trial (AfriDx (RIA2020EF-2918)
- Author contribution statement
- -
- Non-English
- No
- English abstract
- -