Synthesis and cell-free cloning of DNA libraries using programmable microfluidics
- Submitting institution
-
University of Newcastle upon Tyne
- Unit of assessment
- 11 - Computer Science and Informatics
- Output identifier
- 223075-161494-1292
- Type
- D - Journal article
- DOI
-
10.1093/nar/gkv1087
- Title of journal
- Nucleic Acids Research
- Article number
- -
- First page
- 35
- Volume
- 44
- Issue
- 4
- ISSN
- 0305-1048
- Open access status
- Compliant
- Month of publication
- October
- Year of publication
- 2015
- URL
-
http://dx.doi.org/10.1093/nar/gkv1087
- Supplementary information
-
-
- Request cross-referral to
- -
- Output has been delayed by COVID-19
- No
- COVID-19 affected output statement
- -
- Forensic science
- No
- Criminology
- No
- Interdisciplinary
- Yes
- Number of additional authors
-
11
- Research group(s)
-
B - Interdisciplinary Computing and Complex Biosystems (ICOS)
- Citation count
- 10
- Proposed double-weighted
- No
- Reserve for an output with double weighting
- No
- Additional information
- This paper is one of the first (if not the first) to report an integrated computer aided design & manufacturing (CAD/CAM) for combinatorial DNA libraries. It demonstrates that it is possible to do DNA library synthesis at the benchtop. The work uses a biological programming language for defining combinatorial DNA libraries working with off-the-shelf microfluidic devices to achieve benchtop DNA synthesis. The paper was a collaborative effort by FET-Open consortium and builds on previous work that included computational complexity results for DNA libraries assembly (Heuristic for Maximizing DNA Reuse in Synthetic DNA Library Assembly, ACS Synthetic Biology 3, 2014, doi:10.1021/sb400161v).
- Author contribution statement
- -
- Non-English
- No
- English abstract
- -