An Engineered Constitutive Promoter Set with Broad Activity Range for Cupriavidus necator H16
- Submitting institution
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The University of Sheffield
- Unit of assessment
- 12 - Engineering
- Output identifier
- 2781
- Type
- D - Journal article
- DOI
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10.1021/acssynbio.8b00136
- Title of journal
- ACS Synthetic Biology
- Article number
- -
- First page
- 1918
- Volume
- 7
- Issue
- 8
- ISSN
- 2161-5063
- Open access status
- Compliant
- Month of publication
- June
- Year of publication
- 2018
- URL
-
-
- Supplementary information
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-
- Request cross-referral to
- -
- Output has been delayed by COVID-19
- No
- COVID-19 affected output statement
- -
- Forensic science
- No
- Criminology
- No
- Interdisciplinary
- No
- Number of additional authors
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3
- Research group(s)
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-
- Proposed double-weighted
- No
- Reserve for an output with double weighting
- No
- Additional information
- Output from a collaboration between Sheffield, Muenster and a US-based polymer company. These 42 promoters significantly expand the toolbox for engineering Cupriavidus necator. The promoters have been applied to construct the first malonyl-CoA biosensor for C. necator (publication in preparation) and to engineer enhanced glycerol-utilizing strain (International Journal of Molecular Sciences). This work also led to new collaborations with BIOTEC & MTEC (Thai's national research centres), PTTEP (Thailand's largest oil & gas company) and HEL Ltd on biopolymer production, CO2 utilization and gas fermentation, financially supported by BBSRC IAA (R/162832), Royal Society (IES\R3\170129), RAEng (LTSRF1819\15\21) and direct industrial funding (R/162008).
- Author contribution statement
- -
- Non-English
- No
- English abstract
- -