Humanization of bone and bone marrow in an orthotopic site reveals new potential therapeutic targets in osteosarcoma
- Submitting institution
-
Queen Mary University of London
- Unit of assessment
- 12 - Engineering
- Output identifier
- 2594
- Type
- D - Journal article
- DOI
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10.1016/j.biomaterials.2018.04.030
- Title of journal
- Biomaterials
- Article number
- -
- First page
- 230
- Volume
- 171
- Issue
- -
- ISSN
- 0142-9612
- Open access status
- Compliant
- Month of publication
- July
- Year of publication
- 2018
- URL
-
-
- Supplementary information
-
-
- Request cross-referral to
- -
- Output has been delayed by COVID-19
- No
- COVID-19 affected output statement
- -
- Forensic science
- No
- Criminology
- No
- Interdisciplinary
- No
- Number of additional authors
-
16
- Research group(s)
-
-
- Proposed double-weighted
- No
- Reserve for an output with double weighting
- No
- Additional information
- We used a tissue engineering approach to develop a bone tumour platform. We seeded melt electrospun scaffolds with human osteogenic progenitor cells to recreate a bone matrix. In an orthotopic xenograft approach, we implanted these scaffolds at the femur into immuno-deficient animals together with human endothelial cell-seeded hydrogels and osteosarcoma cells. The bone marrow compartment was humanized by irradiation and transplantation of human haematopoietic stem cells. Our new orthotopic platform found new therapeutic targets for the treatment of osteosarcoma. I conceptualized this study and designed the experiments. I performed data analyses. I am second last-named author and edited the manuscript.
- Author contribution statement
- -
- Non-English
- No
- English abstract
- -
