pH plays a role in the mode of action of trimethoprim on Escherichia coli
- Submitting institution
-
Liverpool John Moores University
- Unit of assessment
- 11 - Computer Science and Informatics
- Output identifier
- 2008
- Type
- D - Journal article
- DOI
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10.1371/journal.pone.0200272
- Title of journal
- PLOS ONE
- Article number
- ARTN e0200272
- First page
- -
- Volume
- 13
- Issue
- 7
- ISSN
- 1932-6203
- Open access status
- Compliant
- Month of publication
- July
- Year of publication
- 2018
- URL
-
-
- Supplementary information
-
-
- Request cross-referral to
- -
- Output has been delayed by COVID-19
- No
- COVID-19 affected output statement
- -
- Forensic science
- No
- Criminology
- No
- Interdisciplinary
- Yes
- Number of additional authors
-
4
- Research group(s)
-
-
- Citation count
- 3
- Proposed double-weighted
- No
- Reserve for an output with double weighting
- No
- Additional information
- Trimethoprim is an antibiotic commonly used in the treatment of bladder infections. The off-target effects of trimethoprim have been investigated. E. coli K-12 was challenged with different concentrations of trimethoprim at different pH levels (pH 5 and 7) and analysed by analytical chemistry to produce global snapshots of bacterial phenotypic and untargeted metabolic profiles. Using a metabolomic-based approach coupled with machine learning analysis, this study provided insights on and understanding of trimethoprim’s modes of action fundamental for further drug development and discovery.
- Author contribution statement
- -
- Non-English
- No
- English abstract
- -
