A Protein Chimera Strategy Supports Production of a Model “Difficult-to-Express” Recombinant Target
- Submitting institution
-
The University of Manchester
- Unit of assessment
- 12 - Engineering
- Output identifier
- 83320590
- Type
- D - Journal article
- DOI
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10.1002/1873-3468.13170
- Title of journal
- FEBS Letters
- Article number
- -
- First page
- 2499
- Volume
- 592
- Issue
- 14
- ISSN
- 0014-5793
- Open access status
- Compliant
- Month of publication
- July
- Year of publication
- 2018
- URL
-
-
- Supplementary information
-
-
- Request cross-referral to
- -
- Output has been delayed by COVID-19
- No
- COVID-19 affected output statement
- -
- Forensic science
- No
- Criminology
- No
- Interdisciplinary
- Yes
- Number of additional authors
-
6
- Research group(s)
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C - CEAS
- Proposed double-weighted
- No
- Reserve for an output with double weighting
- No
- Additional information
- This paper defined the molecular relationship between structural features of proteins and restricted secretion, providing predictive and quantifiable means to optimise design of proteins for maximal secretion. In combination with "tug" peptide sequences, this approach refines the potential to manufacture proteins as reagents or therapeutics. This work led to the award of a multi-national (Sweden/UK) £0.4M AZ Research Fellowship, £0.1M EPSRC Researcher in Residence awards (High Value Manufacture and Cell and Gene Therapy Catapults) and invitations to present at international meetings with strong industrial representation (KNect365 Cell Line Development Keynote, San Francisco, June 2019)
- Author contribution statement
- -
- Non-English
- No
- English abstract
- -
