Adaptive optics correction of specimen-induced aberrations in single-molecule switching microscopy
- Submitting institution
-
University of Oxford
- Unit of assessment
- 12 - Engineering
- Output identifier
- 9437
- Type
- D - Journal article
- DOI
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10.1364/OPTICA.2.000177
- Title of journal
- OPTICA
- Article number
- -
- First page
- 177
- Volume
- 2
- Issue
- 2
- ISSN
- 2334-2536
- Open access status
- Out of scope for open access requirements
- Month of publication
- February
- Year of publication
- 2015
- URL
-
-
- Supplementary information
-
-
- Request cross-referral to
- -
- Output has been delayed by COVID-19
- No
- COVID-19 affected output statement
- -
- Forensic science
- No
- Criminology
- No
- Interdisciplinary
- Yes
- Number of additional authors
-
4
- Research group(s)
-
-
- Proposed double-weighted
- No
- Reserve for an output with double weighting
- No
- Additional information
- This is the first demonstration of feedback-based correction of aberrations in super-resolution single molecule fluorescence microscopes. This method of microscopy (as recognised by the Nobel Prize in Chemistry of 2014) is having a large impact on biomedical research, but has been limited to thin specimens like isolated cells. Our developments in adaptive optics are essential in the translation of this method into applications in thicker tissue. The work is referenced by at least 12 other research groups further developing this single molecule adaptive optics technology.
- Author contribution statement
- -
- Non-English
- No
- English abstract
- -