Solvent-selective routing for centrifugally automated solid-phase purification of RNA
- Submitting institution
-
University of Hertfordshire
- Unit of assessment
- 12 - Engineering
- Output identifier
- 13466302
- Type
- D - Journal article
- DOI
-
10.1007/s10404-014-1477-9
- Title of journal
- Microfluidics and Nanofluidics
- Article number
- -
- First page
- 859
- Volume
- 18
- Issue
- 5-6
- ISSN
- 1613-4982
- Open access status
- Out of scope for open access requirements
- Month of publication
- September
- Year of publication
- 2014
- URL
-
-
- Supplementary information
-
-
- Request cross-referral to
- -
- Output has been delayed by COVID-19
- No
- COVID-19 affected output statement
- -
- Forensic science
- No
- Criminology
- No
- Interdisciplinary
- No
- Number of additional authors
-
11
- Research group(s)
-
-
- Proposed double-weighted
- No
- Reserve for an output with double weighting
- No
- Additional information
- This research presents a novel approach for in situ surface modification of a microfluidic chip, allowing for the immobilisation of synthetic enzymes which express polyhistidine tags (His-tag). The novelty in the approach for purification of enzymes lies in the development of the immobilisation and its implementation for continuous biocatalytic synthesis of pharmaceutical compounds with high efficiency and minimum waste. The impact of the research is in the field of bioconversion and biocatalysis. This was a collaborative European project between three institutions (DTU, Denmark & UMPI, Romania & UCL, UK), and it contributed to a successful PhD defence (Dr Gulsim Kulsharova).
- Author contribution statement
- -
- Non-English
- No
- English abstract
- -