LIF-dependent survival of embryonic stem cells is regulated by a novel palmitoylated Gab1 signalling protein
- Submitting institution
-
Coventry University
- Unit of assessment
- 3 - Allied Health Professions, Dentistry, Nursing and Pharmacy
- Output identifier
- 21517626
- Type
- D - Journal article
- DOI
-
10.1242/jcs.222257
- Title of journal
- Journal of Cell Science
- Article number
- jcs222257
- First page
- -
- Volume
- 131
- Issue
- 18
- ISSN
- 0021-9533
- Open access status
- Compliant
- Month of publication
- August
- Year of publication
- 2018
- URL
-
-
- Supplementary information
-
-
- Request cross-referral to
- -
- Output has been delayed by COVID-19
- No
- COVID-19 affected output statement
- -
- Forensic science
- No
- Criminology
- No
- Interdisciplinary
- No
- Number of additional authors
-
17
- Research group(s)
-
-
- Citation count
- 3
- Proposed double-weighted
- No
- Reserve for an output with double weighting
- No
- Additional information
- -
- Author contribution statement
- In this study, I performed experiments, presented and interpreted results. I incubated embryonic stem cells stably-transfected with EGFP or Gab1-EGFP fusion proteins overnight with [3H]palmitate and immunoprecipitated the EGFP proteins. I then separated these on an SDS protein gel, transferred to a nitrocellulose membrane and autoradiographed and probed for EGFP protein. The results demonstrated that Gab1 is palmitoylated. Furthermore, I identified the site of Gab1 palmitoylation by mutating cysteines in the alpha helical domain of the N-terminus. I was also involved in drafting the output, along with critiquing all the figures and data presented.
- Non-English
- No
- English abstract
- -