Analysis of the Hydration Water around Bovine Serum Albumin Using Terahertz Coherent Synchrotron Radiation
- Submitting institution
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The University of Sheffield
- Unit of assessment
- 12 - Engineering
- Output identifier
- 8281
- Type
- D - Journal article
- DOI
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10.1021/jp407410g
- Title of journal
- The Journal of Physical Chemistry A
- Article number
- -
- First page
- 83
- Volume
- 118
- Issue
- 1
- ISSN
- 1089-5639
- Open access status
- Out of scope for open access requirements
- Month of publication
- January
- Year of publication
- 2014
- URL
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- Supplementary information
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- Request cross-referral to
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- Output has been delayed by COVID-19
- No
- COVID-19 affected output statement
- -
- Forensic science
- No
- Criminology
- No
- Interdisciplinary
- No
- Number of additional authors
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5
- Research group(s)
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-
- Proposed double-weighted
- No
- Reserve for an output with double weighting
- No
- Additional information
- Terahertz absorption spectroscopy of aqueous protein deviates significantly from Beers Law indicating a protein hydration layer extending further into the solution than other techniques predict. Collaborating with MedImmune and Diamond Light Source, we measured and independently confirmed this thick fluctuating hydration layer around bovine serum albumin. A novel hydration corrected absorption coefficient was used to rigorously model the results. We demonstrated the sensitivity of terahertz-spectroscopy when studying protein hydration in its natural environment. This complex interaction is significant because water is ubiquitous throughout biology but often ignored. It resulted in a £95k Innovate UK grant (131460: MedImmune, Teraview, Cambridge, Sheffield).
- Author contribution statement
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- Non-English
- No
- English abstract
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