Accelerated directed evolution of dye-decolorizing peroxidase using a bacterial extracellular protein secretion system (BENNY)
- Submitting institution
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The University of Sheffield
- Unit of assessment
- 12 - Engineering
- Output identifier
- 2738
- Type
- D - Journal article
- DOI
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10.1186/s40643-019-0255-7
- Title of journal
- Bioresources and Bioprocessing
- Article number
- 20
- First page
- -
- Volume
- 6
- Issue
- 1
- ISSN
- 2197-4365
- Open access status
- Compliant
- Month of publication
- May
- Year of publication
- 2019
- URL
-
-
- Supplementary information
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-
- Request cross-referral to
- -
- Output has been delayed by COVID-19
- No
- COVID-19 affected output statement
- -
- Forensic science
- No
- Criminology
- No
- Interdisciplinary
- No
- Number of additional authors
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7
- Research group(s)
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-
- Proposed double-weighted
- No
- Reserve for an output with double weighting
- No
- Additional information
- Output from a collaboration between Sheffield and ECUST. It was chosen as the featured article of BIOB June 2019, and translated into Chinese language to benefit more readers. BENNY is being used by researchers in BIOTEC (Thailand) for xylanase engineering. The work has led to a new collaboration with Khalifa University (UAE) on peroxidase engineering for pollutant degradation, and received inquiries from UK and Australia. The BENNY toolbox has now been expanded to include more protein secretion signals (2 publications in preparation) and is applicable to both Gram-positive and Gram-negative bacteria, providing direct benefits to enzyme producers and engineers.
- Author contribution statement
- -
- Non-English
- No
- English abstract
- -